Blogs

The results of the morphological analyses were presented at the 2nd Croatian Microscopy Symposium in Split (November 24-25, 2023). In this study, the application of STEM (scanning transmission electron microscopy) in phytoplankton taxonomy is presented. STEM combines some of the advantages of SEM and TEM methods for easier observation of ultrafine structural features of phytoplankton frustules. In this study, STEM is used to image and morphologically identify the marine diatom genus Pseudo-nitzschia from the central and southern Adriatic Sea using the Tescan FE-SEM /STEM MIRA3 microscope at nanoscale resolution.

8th European Phycological Congress

in Dissemination

August 29, 2023

The first results were presented at the 8th European Phycological Congress in Brest, France (August 20-26, 2023). On the third day of the congress, Tina Bonačić and Jasna Arapov presented their posters with the preliminary results. During our stay, we visited the Roscoff Biological Station, one of the oldest marine biology laboratories in the world, founded in 1872.

Paper published in JMSE

in Dissemination

July 29, 2023

The first results of a comprehensive study on the diversity of Pseudo-nitzschia, combining morphological and molecular analyses, were published in the journal JMSE. The integrated approach resulted in the confirmation of P. brasiliana, P. hasleana, P. galaxiae and P. linea. The findings of P. brasiliana and P. hasleana represent the first confirmation for the Adriatic Sea, while P. galaxiae and P. linea have not yet been previously reported along the eastern Adriatic coast.

BIOCentre workshop

in Dissemination

January 29, 2023

On the 26th and 27th of January 2023 PhD. student Tina Bonačić was attending personalized (individual) workshop in BIOCentre about optimising PCR reaction based on products, analysing gel electoforesis, discusing about electrophotograms and data editing, measuring DNA concentration on NanoDrop sprectrophotometer, and also about design of DNA primers. The workshop included both, lectures and practical work on own samples. Here is a link.

Presenttion of PSEUDOTOX project

in Dissemination

November 29, 2022

Project objectives and some preliminary results were presented in oral presentation at the scientific conference „Croatian Naturalist 31- contribution of naturalists to the development of science in the Split-Dalmatia Country“ in Split on 25-26th November 2022. Here is a link.

Presentation of new Scanning and Transmission Electron Microscope at University of Split

in Lab work

October 26, 2022

On the 26th of October we participated the presentation and the demonstration of new SEM (FEG-SEM JEL JSM 7610F Plus) and the new TEM (JOEL JEM 1400 Flash) at the University of Split.

Presentation in III. Gymnasium Split

in Dissemination

October 14, 2022

On October 14, the doctoral student, Tina Tomašević, gave a lecture to second and third grade students about the ecology and toxicity of species of the genus Pseudo-nitzschia in the coastal waters of the central and southern Adriatic. After the introduction about the HRZZ PSEUDOTOX project, the students had the opportunity, with our help, to determine the species of the genus Pseudo-nitzchia cells photographed on a scanning electron microscope (SEM).

Study visit to Laboratory of Marine Ecology, Campus Enrico Mattei

in Lab work

March 1, 2022

Our Ph.D. students, Mia Pejković and Tina Pavelin, were hosted from 7th to 18th February 2022 in Laboratory of Marine Ecology, Campus Enrico Mattei Urbino by project consultant prof.ssa Antonella Penna. During their stay, they performed molecular analysis on Pseudo-nitzschia spp. cultured strains. The methods used were DNA isolation, quantification, PCR amplification, DNA electrophoresis, and sequence alignment. Also, the Ph.D. students learned more about protocols for culturing phytoplankton species, cell isolation from seawater samples and cell enumeration by light microscopy.

STEM detector

in Lab work

December 16, 2021

The FE-SEM Mira 3 Tescan microscope has been upgraded with a new HADF R- STEM detector, fully funded by Croatia Science Foundation, project PSEUDOTOX. After the successful training held at IOF, the first photos of cultured Pseudo-nitzschia cells are made :).

The new lab, molecular analyses and the sequences....

in Lab work

November 23, 2021

The preliminary clonal Pseudo-nitzschia cultures from field samples collected in the Central and Southern Adriatic Sea has been established. The cultures are further used for species identification by molecular methods in our newly equipped molecular laboratory. After adjustment of DNA isolationmethods and PCR conditions, by shifting polymerase chain reactions and polymerases, we successfully amplificated the gene that encodes for the large ribosomal unit (LSU) in Pseudo-nitzschia. The analyses are going on, and optimization of PCR reaction for ITS region is in progress……

PSEUDOTOX on the Festival of Science 2021

in Dissemination

May 15, 2021

PSEUDOTOX project was presented in the IOF-virtual tour within the Festival of Science 2021. Here is a link.

Kick-off meeting

in Meeting

March 11, 2021

The kick-off meeting took place on March 11th 2021, at the Institute of Oceanography and Fisheries. The working plan and activities for the first year were discussed.

2nd Croatian Microscopy Symposium

8th European Phycological Congress

Paper published in JMSE

BIOCentre workshop

Presenttion of PSEUDOTOX project

Presentation of new Scanning and Transmission Electron Microscope at University of Split

Presentation in III. Gymnasium Split

Study visit to Laboratory of Marine Ecology, Campus Enrico Mattei

STEM detector

The new lab, molecular analyses and the sequences....

PSEUDOTOX on the Festival of Science 2021

Kick-off meeting

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