On June 3, 2024, PhD student Tina Bonačić participated in the Scientific Gathering in Split, organized by the Croatian Society of Biochemistry and Molecular Biology. During the event, Tina presented the main objectives and the accomplished results of the PSEUDOTOX project.
The 15th EDM took place from May 7 th – 9 th , 2024 in the town of Ohrid (North Macedonia). Tina Bonačić and Jasna Arapov presented their posters on the second day of the conference. The presented results included: 1. molecular data based on three markers: ITS, LSU, and rbcL, accompanied by phylogeny analyses and morphological data of established Pseudo-nitzschia cultures (T. Bonačić); 2. the phytoplankton community and Pseudo-nitzschia genus diversity during a two-year field study (J.
The results of the morphological analyses were presented at the 2nd Croatian Microscopy Symposium in Split (November 24-25, 2023). In this study, the application of STEM (scanning transmission electron microscopy) in phytoplankton taxonomy is presented. STEM combines some of the advantages of SEM and TEM methods for easier observation of ultrafine structural features of phytoplankton frustules. In this study, STEM is used to image and morphologically identify the marine diatom genus Pseudo-nitzschia from the central and southern Adriatic Sea using the Tescan FE-SEM /STEM MIRA3 microscope at nanoscale resolution.
The first results were presented at the 8th European Phycological Congress in Brest, France (August 20-26, 2023). On the third day of the congress, Tina Bonačić and Jasna Arapov presented their posters with the preliminary results. During our stay, we visited the Roscoff Biological Station, one of the oldest marine biology laboratories in the world, founded in 1872.
The first results of a comprehensive study on the diversity of Pseudo-nitzschia, combining morphological and molecular analyses, were published in the journal JMSE. The integrated approach resulted in the confirmation of P. brasiliana, P. hasleana, P. galaxiae and P. linea. The findings of P. brasiliana and P. hasleana represent the first confirmation for the Adriatic Sea, while P. galaxiae and P. linea have not yet been previously reported along the eastern Adriatic coast.
On the 26th and 27th of January 2023 PhD. student Tina Bonačić was attending personalized (individual) workshop in BIOCentre about optimising PCR reaction based on products, analysing gel electoforesis, discusing about electrophotograms and data editing, measuring DNA concentration on NanoDrop sprectrophotometer, and also about design of DNA primers. The workshop included both, lectures and practical work on own samples. Here is a link.
Project objectives and some preliminary results were presented in oral presentation at the scientific conference „Croatian Naturalist 31- contribution of naturalists to the development of science in the Split-Dalmatia Country“ in Split on 25-26th November 2022. Here is a link.
On October 14, the doctoral student, Tina Tomašević, gave a lecture to second and third grade students about the ecology and toxicity of species of the genus Pseudo-nitzschia in the coastal waters of the central and southern Adriatic. After the introduction about the HRZZ PSEUDOTOX project, the students had the opportunity, with our help, to determine the species of the genus Pseudo-nitzchia cells photographed on a scanning electron microscope (SEM).
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