Blogs

New paper is published

Thanks to collaboration with colleagues at the Ruđer Bošković Institute, we have published a new paper showing the differential response of plankton (neuston) to changes in the marine environment following the deposition of pyrogenic particles from biomass burning. Read more here.

Presentation of ADRISAAF project results

Project and project results presented in a oral presentation at scientific conference “CROATIAN NATURALISTS 31 - contribution of naturalists to the development of science in the Split-Dalmatia County” held in Split 25.-26. November 2022.

Read more here.

International Symposium on Microbiology Ecology

At the International Symposium on Microbiology Ecology (ISME18) in Lausanne, Switzerland, from 14-19 August 2022, our group members attended and contributed to a Poster session (PS01-Marine Microbial Ecology) with next abstract titles:

Artificial neural network analysis of microbial diversity in the Central and Southern Adriatic Sea (Danijela Šantić)

Seasonal aspects of bacterial diversity along trophic gradient in the eastern Adriatic Sea (Željka Trumbić)

Ecology of Aerobic Anoxygenic phototrophs in the Adriatic Sea (Iva Stojan).

Setting up an experiment...

Testing various conditions for seasonal experiments next year.

ADRISAAF ON THE Science festival

At this year’s Science Festival 2022, our PhD student Iva Stojan participated and presented our project to the citizens and visitors of Split.

New paper is published in Frontiers in Marine Science

We have discovered the existence of different types of microbial food web that develop in certain conditions prevailing in the marine environment. These types of microbial networks differ in their structure, stability, efficiency and functions they perform in marine ecosystems. The results of this research will provide a better understanding of the functioning of marine ecosystems, as well as the changes that can be expected in marine ecosystems as a result of global warming. Read more here.

New paper is out

A new paper in collaboration with colleagues from Czechia and Poland is published. Read more here.

Study visit

We visited our dear colleagues in Michal Kobližek's Laboratory of Anoxygenic Phototrophs in Centre Algatech in Trebon, Czechia. During our amazing two-week stay we analyzed sequencing data from raw sequence reads, thus optimizing bioinformatic pipelines and multivariate analysis needed for our further research. ADRISAAF project activities were presented to their laboratory team as well.

Some nice results

The composite image taken with an epilfluorescence microscope shows AAPs (red), heterotrophic bacteria (blue) and cyanobacteria (green) in the surface layer of the water column.

Project results published in the Scientific Reports

The paper about artificial neural network analysis of microbial diversity is published in the Scientific Reports

ADRISAAF on the Festival of Science

This year in the framework of the Festival of Science virtual tour of the Institute of Oceanography and Fisheries was prepared. See how we presented our work in the Laboratory of Microbiology.

Fieldwork

We have started with our sampling for the survey of whole bacterial community composition (16S), focusing on AAPs (pufM gen).

Presentation of ADRISAAF project to students

Presentation of ADRISAAF project goals and activities to graduate students of "Marine Fisheries" and "Ecology and Protection of the Sea" at the University Department of Marine Studies, University of Split. Students were introduced to the community of aerobic anoxygenic phototrophs and the concept of DNA metabarcoding and its importance in ecological research.

Optimizing DNA isolation protocols

Our first successful outcomes using molecular biology techniques in our lab. Different protocols for DNA isolation and polymerase chain reaction were tested on numerous marine samples. We are currently experimenting with various primer pairs designed for target genes, polymerases and reaction mixtures as well as cycling temperatures to find optimal PCR conditions further to be used in our research.

New equipment

The necessary equipment and chemicals have been procured, and the first analyzes begin.

Our results are published as book chapter in The Montenegrin Adriatic Coast

As part of the book, we were invited to describe our scientific research and include the possible negative effects of pollution on the picoplankton community in the Adriatic Sea.

Šantić D., Vrdoljak Tomaš A., Lušić J. (2020) Spatial and Temporal Patterns of Picoplankton Community in the Central and Southern Adriatic Sea. In: Joksimović A., Đurović M., Zonn I.S., Kostianoy A.G., Semenov A.V. (eds) The Montenegrin Adriatic Coast. The Handbook of Environmental Chemistry, vol 109. Springer, Cham. https://doi.org/10.1007/698_2020_645

First results

Our first results obtained after catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). Various DNA probes for detection of marine bacteria were tested, probes EUB338 (A) for Bacteria and ROS537 (B)for Roseobacter clade are shown here.

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